Expressing:Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF-κB and MAPK signalling transduction through integrin αVβ3
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459-1-AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4°C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF‑κB and MAPK signalling transduction through integrin αVβ3.
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459‐1‐AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4 ̊C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Western Blot:Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF-κB and MAPK signalling transduction through integrin αVβ3
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459-1-AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4°C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF‑κB and MAPK signalling transduction through integrin αVβ3.
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459‐1‐AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4 ̊C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Control:Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF-κB and MAPK signalling transduction through integrin αVβ3
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459-1-AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4°C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF‑κB and MAPK signalling transduction through integrin αVβ3.
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459‐1‐AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4 ̊C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Immunofluorescence:Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF-κB and MAPK signalling transduction through integrin αVβ3
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459-1-AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4°C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF‑κB and MAPK signalling transduction through integrin αVβ3.
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459‐1‐AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4 ̊C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Knockdown:Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF-κB and MAPK signalling transduction through integrin αVβ3
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459-1-AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4°C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF‑κB and MAPK signalling transduction through integrin αVβ3.
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459‐1‐AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4 ̊C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Transfection:Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF-κB and MAPK signalling transduction through integrin αVβ3
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459-1-AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4°C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF‑κB and MAPK signalling transduction through integrin αVβ3.
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459‐1‐AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4 ̊C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Quantitative RT-PCR:Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF-κB and MAPK signalling transduction through integrin αVβ3
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459-1-AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4°C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3-conjugated Affinipure goat anti-rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
Article Title: Rutaecarpine ameliorates osteoarthritis by inhibiting PI3K/AKT/NF‑κB and MAPK signalling transduction through integrin αVβ3.
Article Snippet: The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.The unbound sites on the plates were then blocked using 1% bovine serum albumin for 30 min at normal temperature.. Subsequently, primary antibodies against COL II (1:200; cat. no. 28459‐1‐AP, Proteintech Group, Inc.) and p65 (1:400; cat. no. 8242, Cell Signaling Technology, Inc.) were used to incubate the cells at 4 ̊C overnight.. Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.Following a wash with PBS, the chondrocytes were incubated with Cy3‐conjugated Affinipure goat anti‐rabbit secondary antibody (1:200; cat. no. BA1032, Wuhan Boster Biological Technology, Ltd.) for 1 h at room temperature in the dark.
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